Sharda Singh, PhD | Texas Tech University Health Sciences Center

Sharda Singh, PhD

Sharda Singh

Sharda Singh, PhD

Director of Basic Research, Department of Internal Medicine,
Division of Hematology/Oncology

Curriculum Vitae

 

 

 
  • Sulforaphane attenuates doxorubicin-induced cardiotoxicity without inhibiting anti-cancer activity. Kaplan M, Manavalan M, Hauer-Jensen M, Boerma M, Singh SP. NIH, NIGMS Biennial National IDeA Symposium of Biomedical Research Excellence (NISBRE) Washington DC, June 26-28, 2016.
  • Non-monotonic relationship between tissue levels of 4-hydroxynonenal (4-HNE) and lifespan in C. elegans. McElhanon KE, Bennett KC, Zimniak L, Zimniak P, Singh SP. 18th international meeting of C. elegans, UCLA California, June 22-26, 2011.
  • Singh SP, Ayyadevara S, Zimniak L, Zimniak P. Life span of C. elegans is inversely proportional to the tissue level of the lipid peroxidation product 4-HNE. 16th international meeting of C. elegans, UCLA California, June 24-28, 2009.
  • Awasthi YC, Sharma S, Brown D, Yang Y, Sharma A, Patrick B, Singh SP, Zimniak P, Singh SV and Awasthi S.  Alterations in 4-HNE homeostasis profoundly affect cell cycle signaling events. 2nd Meeting of the HNE club, HNE and lipid peroxidation products: from basic science to medicine. Berlin, July 6-9, 2004.
  • Engle MR, Singh SP, Czernik PJ, Shammas S, Ceci JD, Yang Y, Awasthi S, Awasthi  YC, Zimniak L, Zimniak P. mGsta4 null mouse: Generation and characterization. 2nd Meeting of the HNE club, HNE and lipid peroxidation products: from basic science to medicine. Berlin, July 6-99, 2004.
  • Feng YP, Mondal A, Robinson M, Singh SP, Zimniak P, Beneš H. Isolation and characterization of mutants in the Drosophila Glutathione S-transferase S1 (GST S1) gene. 44th Annual Drosophila Research Conference (USA), March 5-9, 2003.
  • Mondal, A, Singh SP, Feng YP, Beneš H, Zimniak L, Zimniak P. Effect of modifying metabolism of lipid peroxidation on life span. 44th Annual Drosophila Research Conference (USA), March 5-9, 2003.
  • Sawicki R, Singh SP, Zimniak P, Benes H. Cloning and localization of Drosophila melanogaster glutathione transferases (GSTs) belonging to Delta and Epsilon families. 43rd Annual Drosophila Research Conference (USA), April 10-14, 2002.
  • Singh SP, Sawicki R, Zimniak P, Benes H. Characterization of Drosophila melanogaster glutathione transferases (GSTs) involved in metabolism of lipid peroxidation products. 43rd  Annual Drosophila Research Conference (USA), April 10-14, 2002.
  • Singh SP, Janecki A, Srivastava SK, Awasthi S, Awasthi YC, Xia SJ, Zimniak P. Membrane association of murine GSTA4-4, A glutathione transferase that acts on lipid peroxidation products. Society of Toxicology (USA), March 17-21, 2002.
  • Singh SP, Benes H, Coronella JA, Cochrane BJ, Zimniak P. Drosophila melanogaster glutathione transferase conjugating 4-hydroxynonenal. GST 2000: International Conference on Glutathione Transferase, Uppsala, Sweden, May 19-23, 2000.
  • Engle MR, Singh SP, Nanduri B, Ji X, Zimniak P. Invertebrate glutathione transferases conjugating 4-hydroxynonenal: CeGST 5.4 from Caenorhabditis elegans. GST 2000: International Conference on Glutathione Transferase, Uppsala, Sweden, May 19-23, 2000.     
  • Zimniak P, Singh SP, Nanduri B, Awasthi YC, Xiao B, Ji X. Glutathione transferases and the defense against lipid peroxidation and oxidative stress: Insights into the molecular mechanism of cellular protection. 3rd International Symposium on Cell/ Tissue injury and cytoprotection/ Organ protection, USA, January 20, 2000.
  • Zimniak P, Singh SP, Nanduri B, Awasthi YC, Xiao B, Ji X. Catalytic mechanism of mGSTA4-4, An enzyme involved in the response to oxidative stress. Frontiers in Pharmacology and Therapeutics in 21st  Century, India, December 1-4, 1999. 
  • Singh SP, Chabra R, Gupta CM, Srivastava VML. Superoxide burst in peritoneal exudates cells induced by Tuftsin. Indo-French Symposium on Immunomodulation, December 10-13, 1995.
  • Singh SP, Gupta HP, Singh NB, Srivastava VML. Enhancement of effecter activities of macrophages following M. habana vaccination in mice. 12th National Congress of Parasitology , India, January 23-25, 1995.
  • Singh SP, Haq W, Mathur KB, Srivastava VML. Bioactive peptides: Induction of respiratory burst in macrophages.  62nd  Annual Meeting of Society of Biological Chemists, India, December 19-22, 1993.
  • Batra S, Singh SP, Fatima N, Sharma S, Chatterjee RK, Srivastava VML. Effect of 2-2’dicarbomethoxylmino-5, 5’-dibenzimidazolyl ketone on antioxidant defence of Acanthocheilonema viteae and its laboratory host Mastomys natalensis. CSIR Golden Jublee Symposium on Tropical Diseases: Molecular Biology and Control Strategies, India, February 17-20, 1992.
  • Singh SP, Srivastava JK, Katiyar JC, Srivastava VML. Mechanism for the chemoprophylactic action of anthelmintics against Ancylostoma ceylanicum. National symposium on Current Trend in Biotechnology, India, November 28-30, 1990.
  • Srivastava JK, Singh SP, Gupta S, Katiyar JC, Srivastava VML. Metabolic disposition of compound 81/470 and impact on MFO system of hamsters: A study to understand chemoprophylactic action against experimental ancylostomiasis. 10th National Congress of Parasitology, India, March 16-18, 1990.
  • Singh SP, Batra S, Gupta S, Katiyar JC, Srivastava VML. Impact of Ancylostoma ceylanicum infection on ROI metabolizing enzymes of hamster tissues. 10th National Congress of Parasitology, India, March 16-18, 1990.
  • Jain MK, Singh SP, Gupta S, Srivastava VML & Katiyar JC. Effects of anthelmintics on energy metabolism of Cysticercus fasciolaris. 9th National Congress of Parasitology, India, January 29-31, 1989.
  • Batra S, Singh SP, Srivastava VML, Chatterjee RK. Superoxide dismutase and catalase of Dipetalonema viteae. 9th National Congress of Parasitology, India, January 29-31, 1989.
  • Batra S, Singh SP, Chatterjee RK, Srivastava VML. Effect of antifilarial agents on superoxide dismutase and catalase of Mastomys natalensis. 57th Annual Meeting of Society of Biological Chemists, India, October 9-12, 1988.
  • Singh SP, Batra S, Gupta S, Katiyar JC, Srivastava VML. Impact of Ancylostoma ceylanicum infection of leucine amino peptidase activity of Hamster tissue. 57th Annual Meeting of Society of Biological Chemists, India, October 9-12, 1988.
  • Batra S, Chatterji RK, Singh SP. Protein biosynthesis in the filarial parasite Dipetalonema viteae. 56th Annual Meeting of Society of Biological Chemists, India, December 28-30, 1987.
  • Singh SP, Gupta S, Katiyar JC, Srivastava VML. Decarboxylation of amino acids by nematode parasites. 56th Annual Meeting of Society of Biological Chemists, India, December 28-30, 1987. 
 
  • Seawright JW, Sridharan V, Landes RD, Cao M, Singh S.P., Koturbash I, et al. Effects of low-dose oxygen ions and protons on cardiac function and structure in male C57BL/6J mice. Life Sciences in Space Research. (2019);20:72-84.
  • Meda S, Singh S.P., Palade PT, Tonk S, Awasthi S. Oxidative stress in intensive care unit patients: A review of glutathione linked metabolism and lipid peroxidation. The Southwest Respiratory and Critical Care Chronicles. (2019);7:1-29.
  • Bose C, Singh S.P., Igid H, Green WC, Singhal SS, Lee J, et al. Topical 2'-Hydroxyflavanone for Cutaneous Melanoma. Cancers (Basel). (2019);11.
  • Bose, C., Awasthi, S., Sharma, R., Beneš, H., Hauer-Jensen, M., Boerma, M., and Singh, S. P. (2018) Sulforaphane potentiates anticancer effects of doxorubicin and attenuates its cardiotoxicity in a breast cancer model, PloS one 13, e0193918.
  • Awasthi, S., Tompkins, J., Singhal, J., Riggs, A. D., Yadav, S., Wu, X., Singh, S. P., Warden, C., Liu, Z., Wang, J., Slavin, T. P., Weitzel, J. N., Yuan, Y.-C., Awasthi, M., Srivastava, S. K., Awasthi, Y. C., and Singhal, S. S. (2018) Rlip depletion prevents spontaneous neoplasia in TP53 null mice, Proceedings of the National Academy of Sciences.
  • Sims, C. R., Singh, S. P., Mu, S., Gokden, N., Zakaria, D., Nguyen, T. C., and Mayeux, P. R. (2017) Rolipram Improves Outcome in a Rat Model of Infant Sepsis-Induced Cardiorenal Syndrome, Frontiers in pharmacology 8.
  • Marquis, B. J., Louks, H. P., Bose, C., Wolfe, R. R., and Singh, S. P. (2017) A New Derivatization Reagent for HPLC–MS Analysis of Biological Organic Acids, Chromatographia 80, 1723-1732.
  • Wang X, Ding Z, Yang F, Dai Y, Chen P, Theus S, Singh SP, Budhiraja M, Mehta JL. Modulation of Myocardial Injury and Collagen Deposition Following Ischemia/Reperfusion by Linagliptin, Liraglutide, and Both Together. Clin Sci (Lond). 2016.
  • Sridharan V, Thomas CJ, Cao M, Melnyk SB, Pavliv O, Joseph J, Singh SP, Sharma S, Moros EG, Boerma M. Effects of local irradiation combined with sunitinib on early remodeling, mitochondria, and oxidative stress in the rat heart. Radiother Oncol. 2016.
  • Boerma M, Sridharan V, Mao X-W, Nelson GA, Cheema AK, Koturbash I, Singh SP, Tackett AJ, Hauer-Jensen M. Effects of Ionizing Radiation on the Heart. Mutation Research/Reviews in Mutation Research. 2016.
  • Singhal SS, Singh SP, Singhal P, Horne D, Singhal J, Awasthi S. Antioxidant role of glutathione S-transferases: 4-Hydroxynonenal, a key molecule in stress-mediated signaling. Toxicol Appl Pharmacol. 2015;289(3):361-70.
  • Singh P, Sharma R, McElhanon K, Allen CD, Megyesi JK, Benes H, Singh SP. Sulforaphane protects the heart from doxorubicin-induced toxicity. Free Radic Biol Med. 2015;86:90-101.
  • Boerma M, Singh P, Sridharan V, Tripathi P, Sharma S, Singh SP. Effects of Local Heart Irradiation in a Glutathione S-transferase Alpha 4-Null Mouse Model. Radiat Res. 2015;183(6):610-9.
  • Beneš H, Vuong, MK, Boerma M, McElhanon, KE, Siegel, ER, Singh SP.  Protection from oxidative and electrophilic stress in the Gsta4-null mouse heart. Cardiovasc Toxicol. 13:347–356, 2013.
  • McElhanon KE, Bose C, Sharma R, Wu L, Awasthi YC, Singh SP. Gsta4 null mouse embryonic fibroblasts exhibit enhanced sensitivity to oxidants: Role of 4-hydroxynonenal in oxidant toxicity. Open J Apoptosis. 2(1): 1-11, 2013.
  • Singh SP, Niemczyk M, Saini D, Sadovov V, Zimniak L, Zimniak P. Disruption of the mGsta4 gene increases life span of C57BL mice. J Gerontol Ser. A: Biol Sci & Med Sci. 65(1):14-23, 2010.
  • Singh SP, Zimniak L, Zimniak P. The human hGSTA5 gene encodes an enzymatically active protein. Biochim Biophys Acta. 1800(1): 16-22, 2009.
  • Singh SP, Niemczyk M, Zimniak L, Zimniak P. Fat accumulation in Caenorhabditis elegans triggered by the electrophilic lipid peroxidation product 4-hydroxynonenal (4-HNE). Aging. 1: 68-80, 2009.
  • Singh SP, Niemczyk M, Saini D, Awasthi YC, Zimniak L, Zimniak P. Role of the electrophilic lipid peroxidation product 4-hydroxynonenal in the development and maintenance of obesity in mice. Biochemistry. 47(12):3900-3911, 2008.
  • Ayyadevara S, Dandapat A, Singh SP, Siegel ER, Shmookler Reis RJ, Zimniak L, Zimniak P. Life span and stress resistance of Caenorhabditis elegans are differentially affected by glutathione transferases metabolizing 4-hydroxynon-2-enal. Mech Ageing Dev. 128(2):196-205, 2007.
  • Ayyadevara S, Dandapat A, Singh SP, Benes H, Zimniak L, Reis RJ, Zimniak P. Lifespan extension in hypomorphic daf-2 mutants of Caenorhabditis elegans is partially mediated by glutathione transferase CeGSTP2-2. Aging Cell. 4(6):299-307, 2005.
  • Gong H, Singh SV, Singh SP, Mu Y, Lee JH, Saini SP, Toma D, Ren S, Kagan VE, Day BW, Zimniak P, Xie W. Orphan nuclear receptor pregnane X receptor sensitizes oxidative stress responses in transgenic mice and cancerous cells. Mol Endocrinol. 20(2):279-290, 2006.
  • Ayyadevara S, Engle MR, Singh SP, Dandapat A, Lichti CF, Benes H, Shmookler Reis RJ, Liebau E, Zimniak P. Lifespan and stress resistance of Caenorhabditis elegans are increased by expression of glutathione transferases capable of metabolizing the lipid peroxidation product 4-hydroxynonenal. Aging Cell. 4(5):257-271, 2005.
  • Singh SP, Chen T, Chen L, Mei N, McLain E, Samokyszyn V, Thaden JJ, Moore MM, Zimniak P. Mutagenic effects of  4-hydroxynonenal triacetate, a chemically protected form of the lipid peroxidation product  4-hydroxynonenal, as assayed in L5178Y/Tk+/- mouse lymphoma cells. JPET. 313:855–861, 2005.
  • Sharma S, Brown, D, Awasthi S, Yang Y, Sharma A, Patrick B, Saini MK, Singh SP, Zimniak P, Singh SV, Awasthi YC. Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells. Eur J Biochem. 271: 1-12, 2004.
  • Engle MR, Singh SP, Czernik PJ, Gadd, D, Montague DC, Ceci JD, Yang Y, Awasthi S, Awasthi YC, Zimniak P. Physiological role of mGSTA4-4, a glutathione S-transferase metabolizing 4-hydroxynonenal: generation and analysis of mGsta4 null mouse.  Toxicol and App Pharmacol. 194: 296-308, 2004.
  • Sawicki R, Singh SP, Mondal AK, Benes H, Zimniak P. Cloning, expression, and biochemical characterization of one Epsilon-class (GST-3) and ten Delta class (GST-1) glutathione S-transferases from Drosophila melanogaster, and identification of additional nine members of the Epsilon class. Biochem J. 370: 661-669, 2003.
  • Singh SP, Janecki AJ, Srivastava SK, Awasthi S, Awasthi YC, Xia PJ, Zimniak P. Membrane association of glutathione S-transferase mGSTA4-4, an enzyme that metabolizes lipid peroxidation products. J Biol Chem. 277(6): 4232-9, 2002.
  • Bose C, Guo J, Zimniak L, Srivastava SK, Singh SP, Zimniak P, Singh SV.  Critical role of allyl groups and disulfide chain in induction of Pi class glutathione transferase in mouse tissues in vivo by diallyl disulfide, a naturally occurring chemo preventive agent in garlic. Carcinogenesis. 23(10): 1661-1665, 2002.
  • Cheng JZ, Yang Y, Singh SP, Singhal SS, Awasthi S, Pan SS, Singh SV, Zimniak P, Awasthi YC. Two distinct 4-hydroxynonenal metabolizing glutathione S-transferase isozymes are differentially expressed in human tissues. Biochem Biophys Res Commun. 282(5): 1268-74, 2001.
  • Singh SP,  Coronella JA, Benes H, Cochrane BJ, Zimniak P. Catalytic function of Drosophila melanogaster glutathione S-transferase DmGSTS1-1 (GST-2) in conjugation of lipid peroxidation end products. Eur. J. Biochem. 268: 1-13, 2001.
  • Engle MR, Singh SP, Nanduri B, Ji X, Zimniak P. Invertebrate glutathione transferases conjugating 4-hydroxynonenal: CeGST 5.4 from Caenorhabditis elegans. Chemico-Biological Interactions. 133:244-248, 2001.
  • Singh SP, Benes H, Coronella JA, Cochrane BJ, Zimniak P. Drosophila melanogaster glutathione transferase conjugating 4-hydroxynonenal. Chemico-Biological Interactions. 133: 224-227, 2001.
  • Xiao B, Singh SP, Nanduri B, Awasthi YC, Zimniak P, Ji X. Crystal structure of murine glutathione S-transferase in complex with a glutathione conjugate of 4-hydroxynon-2-enal in one subunit and glutathione in the other: evidence of signaling across the dimer interface. Biochemistry. 38:11887-11894, 1999.
  • Srivastava JK, Singh SP, Katiyar JC, Srivastava VML. Effect of methyl[5[(-4(2-pyridinyl)-l-piperazinyl] carbonyl-H-benzimidazole-2yl]carbamate on antioxidant and mixed function oxidase system in hamster liver. Med Sci Res. 22: 197-199, 1994.
  • Singh SP, Srivastava JK, Katiyar JC, Chatterjee RK, Srivastava VML. Potential of amino acids to support maintenance of A. ceylanicum in vitro. Indian J Exp Biol. 34: 609- 611, 1994.
  • Singh SP, Srivastava JK, Katiyar JC, Srivastava VML. Role of reactive oxygen species in the chemoprophylactic action of methyl [5-[[4-(2-pyridinyl)-l- piperazinyl] carbonyl] 1H-benzimidazole-2yl]carbamate in hamster against Ancylostoma ceylanicum. Biochem Pharmacol. 47: 2253-2257, 1994.   
  • Srivastava JK, Singh SP, Gupta S, Katiyar JC, Srivastava VML. Metabolic disposition of methyl [5-[[4-(2-pyridinyl)-l-piperazinyl)carbonyl]-1H-benzimidazole-2yl]carbamate in hamster: A study to understand chemoprophylactic action against experimental Ancylostomiasis. Indian J Exp Biol. 32: 533-539, 1994.
  • Batra S, Singh SP, Fatma S, Sharma S, Chatterjee RK, Srivastava VML. Effect of 2,2’-dicarbomethoxylamino-5, 5’-dibenzimidazolyl ketone on antioxidant defence of A. viteae and its laboratory host M. natalensis. Biochem Pharmacol. 44: 727-731, 1992.
  • Singh SP, Chhabra R, Srivastava VML. Respiratory burst in peritoneal cell in response to modified tuftsin. Experientia. 48: 994-996, 1992.
  • Singh SP, Batra S, Gupta S, Katiyar JC, Srivastava VML. Effect of A. celyanicum on antioxidant system in hamster tissue. Med Sci Res. 20: 605-608, 1992.
  • Singh SP, Gupta S, Katiyar JC, Srivastava VML. On the potential of amino acids to support survival and energy status of N. brasiliensis in vitro. International J Parasitol. 22:131-133, 1992.
  • Singh SP, Katiyar JC, Srivastava VML. Enzymes of tricarboxylic acid cycle in A. ceylanicun and N. brasiliensis. J Parasitol. 178:24-29, 1992.
  • Jain MK, Singh SP, Gupta S, Katiyar JC, Srivastava VML. Alteration in the metabolic activity of C. fasciolaris following anthelmintic treatment in situ. Indian J Exp Biol. 30:320-23, 1989.
  • Singh SP, Gupta S, Katiyar JC, Srivastava VML. Amino acid decarboxylases of A. ceylanicum and N. brasiliensis. Current Science. 58:1353-1356, 1989.
  • Batra S, Singh SP, Gupta S, Katiyar JC, Srivastava VML. Xanthine oxidase, superoxide dismutase, catalase, and lipid peroxidation in M. natalensis infected with D. viteae. Indian J  Exp Biol. 27:1067-1070, 1989.
  • Batra S. Singh SP, Gupta S, Katiyar JC, Srivastava VML. Reactive oxygen intermediates metabolizing enzymes in A. ceylanicum and N. brasiliensis. Free Radicals in Biol and Med. 8:271-274, 1989.
  • Singh SP, Batra S, Gupta S, Katiyar JC, Srivastava VML. Effect of A. ceylanicum infection in hamster on enzymes, which metabolize oxygen intermediates. Med Sci Res. 17: 493-495, 1989.
  • Singh SP., Batra S, Gupta S, Katiyar JC, Chatterjee RK, Srivastva VML. Leucine amino peptidase in host tissues during A. ceylanicum and D. viteae infection. Indian J Parasitol. 13: 187-190, 1989.
  • Singh SP, Srivastva VML. Amino acid metabolism in Setaria cervi: decarboxylation reaction. Indian J Parasitol. 12: 297-299, 1988.