Center for Tumor Immunology and Targeted Cancer Therapy

Mission:

The mission of the Center is to improve cancer patient care through the development and implementation of novel immunotherapies and targeted therapies for cancer.

Vision:

To grow basic, translational, and clinical research in tumor immunology, cancer biology and personalized medicine at the School of Pharmacy through the establishment of a team of multidisciplinary and interdepartmental investigators working in a highly collaborative and supportive environment.

Analytical Instrumentation:

Multispectral imaging flow cytometer with ability to locate and quantitate the distribution of signals on or within cells and between cells in cell conjugates.

Lasers:
405nm Violet Laser
488nm Sapphire Laser
658nm Red Laser
785nm

Create high-quality digital slides with the ultra-compact Aperio CS2 image capture device – from your desktop. With a five-slide capacity and 20X and 40x magnification capabilities, the Aperio CS2 is a highly reliable workhorse for medium-volume sites.

Aperio CS2 is the ideal tool for preparing slides and data to be shared with other team members for collaboration. Remote assessment of digital slides can improve study turnaround time and ease of access for team members.

A compact, small footprint, high image quality whole slide line scanning instrument, Aperio CS2 occupies only 1.6 square feet of bench top space.

Key Features:

External sharing and collaboration (institution to institution)
Internal sharing (Hub & Spoke Network)and collaboration
eIHC image analysis
Contract research
Tissue microarrays

The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa analyzer can be configured with up to 4 lasers—blue, red, violet, and UV—which enables the detection of up to 18 colors simultaneously. The BD LSRFortessa may be upgraded subsequently with additional or new lasers from BD, as future requirements dictate.

Lasers:

405 Violet Laser
488 Blue Laser
640 Red Laser

Key Features:

The fluidics system is pressure driven. Hydrodynamic focusing forces sample cells through the cuvette flow cell, where they are interrogated. The flow cell is in fixed alignment with the laser and gel-coupled to the collection optics. This design ensures that the laser is precisely focused on the sample stream and the maximum amount of emitted light can be collected for added sensitivity in multicolor applications. Fixed alignment also minimizes startup time, improves experiment-to-experiment reproducibility, and enables automated daily quality control.
The optics system consists of laser excitation optics that illuminate cells in the sample, and collection optics that direct light scatter and fluorescence signals through spectral filters to detectors. Innovative designs for both the excitation optics and collection optics in BD LSRFortessa systems reduce excitation losses and optimize collection efficiency for increased sensitivity and resolution.

The BD FACSAria cell sorter sets a new standard for high-performance flow cytometry. Based on an entirely new design in instrumentation, the BD FACSAria instrument is the first benchtop sorter that incorporates a fixed-alignment cuvette flow cell. The numerous technological advances embodied in the BD FACSAria cell sorter reduce the cost of owning a highspeed sorter and accelerate research by providing unparalleled, easy-to-use, high-performance sorting and analysis.

With 3 lasers, it can detect up to 11 fluorochromes, for a total of 13 parameters:

488 nm: 7 detectors (SSC + 6 fluorochromes)
633 nm: 3 detectors
405 nm (violet): 2 detectors

Key Features:

High-speed cell sorting
3 air-cooled lasers (blue: 488, red: 633, and violet: 407 nm)
Digital acquisition rates of up to 70,000 events/second
Multicolor analysis (up to 9 colors)
Two- and four-way bulk sorting into tubes and 96-well plates
Optical filters to detect fluorophores in the following nine channels:
Digital Fluorescence-activated cell sorter

The A1 is Nikon's powerful fully-automated confocal imaging system, capable of capturing high-quality confocal images of cells and molecular events at high speed and enhanced sensitivity. Ideal for facilities with a broad range of users, the A1 has been designed with groundbreaking new optical and electronic technology innovations to provide unprecedented system quality and flexibility. High precision motorized-focus, and image capture in 3D and many more features.

Equipped with 4 lasers:

405
488
561
635nm.

Key Features:

A high-speed resonant scanner allows imaging of intracellular dynamics at 30 frames per second (Fps). Moreover, image acquisition of 420 fps is also possible.
Simultaneous imaging and photo activation with the proprietary hybrid scanner reveal intermolecular interaction. Analysis software for FRAP and FRET is provided standard.
Fast spectral image acquisition for 32 channels at a maximum of 24 fps (512 x 32 pixels) is possible. New real-time spectral unmixing and the V-filtering function expand the range of use of spectral images.
Fluorescence efficiency is increased by 30 percent, and S/N ratio of images is also increased. With diverse new technologies such as the VAAS pinhole unit, superior image quality has been achieved.